fluorescence activated cell sorting facs buffer (Thermo Fisher)
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Thermo Fisher
fluorescence activated cell sorting facs buffer
Fluorescence Activated Cell Sorting Facs Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescence+activated+cell+sorting+facs+buffer/PHOSPHATE+BUFFERED+SALINE+PBS/pm41917203-192-5-31
Average 99 stars, based on 1 article reviews
Fluorescence Activated Cell Sorting Facs Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescence+activated+cell+sorting+facs+buffer/PHOSPHATE+BUFFERED+SALINE+PBS/pm41917203-192-5-31
Average 99 stars, based on 1 article reviews
fluorescence activated cell sorting facs buffer - by Bioz Stars,
2026-09
99/100 stars
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Staining:Article Title: Protective Role for Itaconate During Inhaled Allergen Challenge. Article Snippet: The left lung lobe was finely chopped and incubated with cRPMI supplemented with 0.15 mg/mL Collagenase D (Roche Diagnostics) and 25 μg/mL DNase I (Roche Diagnostics) at 37°C for 1 h. The recovered cells were passed through a 70 μm filter to obtain a single- cell suspension; erythrocytes were lysed using a 55 mM NH4Cl/10 mM KMCO3/0.1 mM Na2EDTA lysis buffer, pelleted, and resuspended in cRPMI. .. BAL and lung cells were stained with near- infrared fixable live/ dead dye (1:1000, Thermo Fisher) before staining for extracellular antigens in Article Title: Immunosuppressive macrophages determine the effect of cellular senescence on tumor progression Article Snippet: .. Cells were blocked in Article Title: GPR25 promotes the formation of lung and liver tissue-resident memory CD8 T cells Article Snippet: To isolate lymphocytes from lung tissues, tissue was cut into small pieces and dispersed for 15 min with 1 ml of digestion buffer (RPMI-1640 (Gibco) + 10% FBS (Gibco), supplemented with 200 U/ml Type 2 Collagenase (Worthington) and 15 μg/ml DNase (Sigma)) while shaking at 200 rpm for 15–20 min at 37°C. .. Single-cell suspensions were prepared and surface stained in Fluorescence:Article Title: Protective Role for Itaconate During Inhaled Allergen Challenge. Article Snippet: The left lung lobe was finely chopped and incubated with cRPMI supplemented with 0.15 mg/mL Collagenase D (Roche Diagnostics) and 25 μg/mL DNase I (Roche Diagnostics) at 37°C for 1 h. The recovered cells were passed through a 70 μm filter to obtain a single- cell suspension; erythrocytes were lysed using a 55 mM NH4Cl/10 mM KMCO3/0.1 mM Na2EDTA lysis buffer, pelleted, and resuspended in cRPMI. .. BAL and lung cells were stained with near- infrared fixable live/ dead dye (1:1000, Thermo Fisher) before staining for extracellular antigens in Article Title: Enterococcus faecalis persists and replicates intracellularly within neutrophils. Article Snippet: .. The enzymatic solution was then aspirated, and cells were blocked in 500 μL of Article Title: Key features of the innate immune response is mediated by the immunoproteasome in microglia. Article Snippet: .. Cells were then dislodged from the culture surface by scraping in Article Title: Enterococcus faecalis persists and replicates intracellularly within neutrophils Article Snippet: .. The enzymatic solution was then aspirated, and cells were blocked in 500 μL of Article Title: Immunosuppressive macrophages determine the effect of cellular senescence on tumor progression Article Snippet: .. Cells were blocked in Article Title: GPR25 promotes the formation of lung and liver tissue-resident memory CD8 T cells Article Snippet: To isolate lymphocytes from lung tissues, tissue was cut into small pieces and dispersed for 15 min with 1 ml of digestion buffer (RPMI-1640 (Gibco) + 10% FBS (Gibco), supplemented with 200 U/ml Type 2 Collagenase (Worthington) and 15 μg/ml DNase (Sigma)) while shaking at 200 rpm for 15–20 min at 37°C. .. Single-cell suspensions were prepared and surface stained in Article Title: Key features of the innate immune response is mediated by the immunoproteasome in microglia Article Snippet: .. Cells were then dislodged from the culture surface by scraping in Article Title: Blood vessel-resident macrophages safeguard blood and vessel integrity in zebrafish. Article Snippet: After removal of medium, embryos were put Nature Immunology Article https://doi.org/10.1038/s41590-026-02481-y in TrypLE (ThermoFisher, 12563011) for 45 min at 32 °C with vigorous shaking. .. Cells were then washed with FACS:Article Title: Protective Role for Itaconate During Inhaled Allergen Challenge. Article Snippet: The left lung lobe was finely chopped and incubated with cRPMI supplemented with 0.15 mg/mL Collagenase D (Roche Diagnostics) and 25 μg/mL DNase I (Roche Diagnostics) at 37°C for 1 h. The recovered cells were passed through a 70 μm filter to obtain a single- cell suspension; erythrocytes were lysed using a 55 mM NH4Cl/10 mM KMCO3/0.1 mM Na2EDTA lysis buffer, pelleted, and resuspended in cRPMI. .. BAL and lung cells were stained with near- infrared fixable live/ dead dye (1:1000, Thermo Fisher) before staining for extracellular antigens in Article Title: Enterococcus faecalis persists and replicates intracellularly within neutrophils. Article Snippet: .. The enzymatic solution was then aspirated, and cells were blocked in 500 μL of Article Title: Key features of the innate immune response is mediated by the immunoproteasome in microglia. Article Snippet: .. Cells were then dislodged from the culture surface by scraping in Article Title: Enterococcus faecalis persists and replicates intracellularly within neutrophils Article Snippet: .. The enzymatic solution was then aspirated, and cells were blocked in 500 μL of Article Title: Immunosuppressive macrophages determine the effect of cellular senescence on tumor progression Article Snippet: .. Cells were blocked in Article Title: GPR25 promotes the formation of lung and liver tissue-resident memory CD8 T cells Article Snippet: To isolate lymphocytes from lung tissues, tissue was cut into small pieces and dispersed for 15 min with 1 ml of digestion buffer (RPMI-1640 (Gibco) + 10% FBS (Gibco), supplemented with 200 U/ml Type 2 Collagenase (Worthington) and 15 μg/ml DNase (Sigma)) while shaking at 200 rpm for 15–20 min at 37°C. .. Single-cell suspensions were prepared and surface stained in Article Title: Key features of the innate immune response is mediated by the immunoproteasome in microglia Article Snippet: .. Cells were then dislodged from the culture surface by scraping in Article Title: Blood vessel-resident macrophages safeguard blood and vessel integrity in zebrafish. Article Snippet: After removal of medium, embryos were put Nature Immunology Article https://doi.org/10.1038/s41590-026-02481-y in TrypLE (ThermoFisher, 12563011) for 45 min at 32 °C with vigorous shaking. .. Cells were then washed with Single Cell:Article Title: GPR25 promotes the formation of lung and liver tissue-resident memory CD8 T cells Article Snippet: To isolate lymphocytes from lung tissues, tissue was cut into small pieces and dispersed for 15 min with 1 ml of digestion buffer (RPMI-1640 (Gibco) + 10% FBS (Gibco), supplemented with 200 U/ml Type 2 Collagenase (Worthington) and 15 μg/ml DNase (Sigma)) while shaking at 200 rpm for 15–20 min at 37°C. .. Single-cell suspensions were prepared and surface stained in Saline:Article Title: Blood vessel-resident macrophages safeguard blood and vessel integrity in zebrafish. Article Snippet: After removal of medium, embryos were put Nature Immunology Article https://doi.org/10.1038/s41590-026-02481-y in TrypLE (ThermoFisher, 12563011) for 45 min at 32 °C with vigorous shaking. .. Cells were then washed with Concentration Assay:Article Title: Blood vessel-resident macrophages safeguard blood and vessel integrity in zebrafish. Article Snippet: After removal of medium, embryos were put Nature Immunology Article https://doi.org/10.1038/s41590-026-02481-y in TrypLE (ThermoFisher, 12563011) for 45 min at 32 °C with vigorous shaking. .. Cells were then washed with |